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KMID : 1190620070030010009
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Journal of the Korean Wound Care Society
2007 Volume.3 No. 1 p.9 ~ p.13
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Preadipocyte Adhesion and Proliferation on Biodegradable Polymer Surfaces
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Choi Yun-Seok
Rhie Jong-Won
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Abstract
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A variety of materials have been explored as scaffolding for tissue engineering. Biodegradable natural materials, collagen and chitosan, and synthetic biodegradable polymers, poly-L-lactide (PLLA), poly (lactide-co-glycolide) (PLGA), have been explored more recently as scaffolds for tissue-engineered transplants. We studied the effect of adhesion and proliferation of preadipocyte on two dimensional synthetic bioidegradable polymer films depend on chemical composition of these material. We used coverglass as a control and PLLA, 50：50 PLGA, 75：25 PLGA, and 85：15 PLGA as experimental groups. Attachment of cells was evaluated at 2, 4, 8, 12, and 24 hr after seeding of 1×104cells/well, and proliferation of cells evaluated at 1 and 3 day of culture time by DNA assay. These results are as follows. 1. In all groups, attachment of cells was begun after 2 hr of culture. 2. After 2 hr of culture, the maximal percentage of preadipocyte attached to control of 49%, and minimal attachment of 10% was found on 50：50 PLLA group. 3. After 24 hr of culture, almost of cells attached to the groups ranged from 91% (control), maximally, to 81% (PLLA group), minimally. 4. After 1 day of culture, the DNA amount of control was the highest (14.6±2.4) and the PLLA group was the least (5.9±2.1) among the groups. 5. After 3 day of culture, the DNA amount of 85：15 PLGA was the highest (15.2±2.4) and the 50：50 PLGA group was the least (8.5±1.5) among the groups. These results represent that no significant differences of cell attachment among the groups after 12 hr of culture was found and the proliferation of cells was highest on the 85：15 PLGA group. This study suggests that 85：15 PLGA is the most suitable material for attachment and growth of the preadipocytes among the other biodegradable synthetic polymers. (J Korean Wound Care Soc 2007;3:9-13)
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KEYWORD
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Cells, Cultured, Surface property, Preadipocyte, Biodegradable polymer, Cell proliferaion
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